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KMID : 0380620190510030272
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 3 p.272 ~ p.277
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Medium-chain fatty acid enriched-diacylglycerol (MCE-DAG) accelerated cholesterol uptake and synthesis without impact on intracellular cholesterol level in HepG2
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Kim Hyun-Kyung
Choi Jong-Hun
Kim Hun-Jung
Kim Woo-Ki
Go Gwang-Woong
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Abstract
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The effects of medium-chain enriched diacylglycerol (MCE-DAG) oil on hepatic cholesterol homeostasis were investigated. HepG2 hepatocytes were treated with either 0.5, 1.0, or 1.5 μg/mL of MCE-DAG for 48 h. There was no evidence of cytotoxicity by MCE-DAG up to 1.5 μg/mL. The level of proteins for cholesterol uptake including CLATHRIN and LDL receptor increased by MCE-DAG in a dose-dependent manner (p<0.05). Furthermore, proprotein convertase subtilisin/kexin type 9, an inhibitor of LDLR, was dose-dependently diminished (p<0.05), indicating cholesterol clearance raised. MCE-DAG significantly increased 3-hydroxy-3-methylglutaryl-coenzyme A reductase and acetyl-CoA acetyltransferase2 (p<0.05), required for cholesterol synthesis, and their transcriptional regulator sterol regulatory elementbinding protein2 (p<0.05). These findings suggest that given conditions of prolonged sterol fasting in the current study activated both hepatic cholesterol synthesis and clearance by MCE-DAG. However, total intracellular level of cholesterol was not altered by MCE-DAG. Taken together, MCE-DAG has the potential to prevent hypercholesterolemia by increasing hepatic cholesterol uptake without affecting intracellular cholesterol level.
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KEYWORD
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cholesterol clearance, cholesterol homeostasis, cholesterol synthesis, medium-chain fatty acid enricheddiacylglycerol, structured lipid
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